What are the key techniques and methodologies you are familiar with in molecular biology?

Sample interview questions: What are the key techniques and methodologies you are familiar with in molecular biology?

Sample answer:

  • Gel electrophoresis: This technique is used to separate DNA, RNA, and proteins based on their size and charge. It is a fundamental technique in molecular biology and is used in a wide variety of applications, such as DNA sequencing, genotyping, and gene expression analysis.

  • PCR (polymerase chain reaction): This technique is used to amplify a specific region of DNA. It is a powerful tool that has revolutionized molecular biology and is used in a wide variety of applications, such as DNA sequencing, genotyping, gene expression analysis, and genetic engineering.

  • DNA sequencing: This technique is used to determine the order of nucleotides in a DNA molecule. It is a fundamental technique in molecular biology and is used in a wide variety of applications, such as gene identification, genotyping, and evolutionary studies.

  • Gene expression analysis: This technique is used to measure the amount of RNA or protein that is produced from a gene. It is used to study gene regulation and to identify genes that are involved in various diseases.

  • Genetic engineering: This technique is used to modify the genetic material of an organism. It is used to create genetically modified organisms (GMOs), which are used in a variety of applications, such as agriculture, medicine, and industrial biotechnology.

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