Sample interview questions: Can you describe any experience you have with studying the biochemistry of RNA editing and modifications?
Sample answer:
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Experience with RNA Editing Techniques:
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Performed enzymatic and chemical RNA editing reactions in vitro using various editing enzymes and reagents, including ADARs, ALKBHs, and CRISPR-Cas systems.
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Developed and optimized protocols for site-specific RNA editing in vitro and in cellular systems.
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Conducted experiments to investigate the efficiency, specificity, and mechanisms of RNA editing reactions.
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Expertise in RNA Modification Analysis:
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Utilized high-throughput sequencing techniques, such as RNA-seq and bisulfite sequencing, to analyze RNA modifications on a genome-wide scale.
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Developed and applied bioinformatics tools for the analysis of RNA modification data, including sequence alignment, peak calling, and differential modification analysis.
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Conducted experiments to identify and characterize novel RNA modifications and their biological significance.
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Understanding of RNA Editing and Modification Pathways:
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Possess a comprehensive understanding of the various types of RNA editing and modification pathways, including ADAR-mediated RNA editing, ALKBH-mediated RNA demethylation, and CRISPR-Cas-mediated RNA editing.
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Stay updated with the latest research advancements in the field of RNA editing and modification through literature reviews, conferences, and seminars.
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Experience with Cellular and Molecular Biology Techniques:
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Conducted experiments involving cell culture… Read full answer
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