Sample interview questions: Can you discuss any experience you have with next-generation sequencing technologies?
Sample answer:
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Experience with Illumina platforms:
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Proficient in library preparation using Illumina TruSeq and Nextera XT kits
- Operated Illumina HiSeq and MiSeq instruments for whole-genome sequencing, exome sequencing, and RNA sequencing
- Performed data analysis using Illumina’s BaseSpace and DRAGEN pipelines
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Developed custom bioinformatics workflows for variant calling, gene expression analysis, and microbiome analysis
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Experience with Ion Torrent platforms:
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Conducted library preparation using Ion Torrent AmpliSeq and Ion AmpliSeq Exome kits
- Operated Ion Torrent PGM and S5 instruments for targeted sequencing and whole-genome sequencing
- Performed data analysis using Ion Torrent’s Torrent Suite and VariantCaller pipelines
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Developed custom bioinformatics workflows for variant calling and gene expression analysis
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Experience with Oxford Nanopore platforms:
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Prepared libraries using Oxford Nanopore Ligation Sequencing Kit and Rapid Barcoding Kit
- Operated Oxford Nanopore MinION and GridION sequencers for long-read sequencing
- Performed data analysis using Oxford Nanopore’s Guppy and EPI2ME pipelines
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Developed custom bioinformatics workflows for variant calling, structural variant analysis, and RNA sequencing
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Experience with Single-Cell Sequencing:
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Utilized microfluidic platforms, such as the 10x Genomics Chromium Controller, to generate single-cell suspensions
- Performed single-cell library preparation using 10x Genomics Single Cell 3′ Library Kit and Smart-seq2 protocol
- Sequenced single-cell libraries on Illumina HiSeq and NovaSeq platforms
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Analyzed single-cell sequencing data using specialized software, such as Seurat and Scanpy, to identify cell types, study gene expression patterns, and reconstruct … Read full answer