Can you discuss any experience you have with next-generation sequencing technologies?

Sample interview questions: Can you discuss any experience you have with next-generation sequencing technologies?

Sample answer:

  • Experience with Illumina platforms:

  • Proficient in library preparation using Illumina TruSeq and Nextera XT kits

  • Operated Illumina HiSeq and MiSeq instruments for whole-genome sequencing, exome sequencing, and RNA sequencing
  • Performed data analysis using Illumina’s BaseSpace and DRAGEN pipelines
  • Developed custom bioinformatics workflows for variant calling, gene expression analysis, and microbiome analysis

  • Experience with Ion Torrent platforms:

  • Conducted library preparation using Ion Torrent AmpliSeq and Ion AmpliSeq Exome kits

  • Operated Ion Torrent PGM and S5 instruments for targeted sequencing and whole-genome sequencing
  • Performed data analysis using Ion Torrent’s Torrent Suite and VariantCaller pipelines
  • Developed custom bioinformatics workflows for variant calling and gene expression analysis

  • Experience with Oxford Nanopore platforms:

  • Prepared libraries using Oxford Nanopore Ligation Sequencing Kit and Rapid Barcoding Kit

  • Operated Oxford Nanopore MinION and GridION sequencers for long-read sequencing
  • Performed data analysis using Oxford Nanopore’s Guppy and EPI2ME pipelines
  • Developed custom bioinformatics workflows for variant calling, structural variant analysis, and RNA sequencing

  • Experience with Single-Cell Sequencing:

  • Utilized microfluidic platforms, such as the 10x Genomics Chromium Controller, to generate single-cell suspensions

  • Performed single-cell library preparation using 10x Genomics Single Cell 3′ Library Kit and Smart-seq2 protocol
  • Sequenced single-cell libraries on Illumina HiSeq and NovaSeq platforms
  • Analyzed single-cell sequencing data using specialized software, such as Seurat and Scanpy, to identify cell types, study gene expression patterns, and reconstruct … Read full answer

    Source: https://hireabo.com/job/5_1_20/Biotechnologist

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