Sample interview questions: Can you discuss any experience you have in analyzing and interpreting transcriptome data from non-model organisms using bioinformatics tools?
Sample answer:
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Experience in analyzing and interpreting transcriptome data from non-model organisms using bioinformatics tools:
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Conducted a transcriptome analysis of a novel extremophilic bacterium using RNA-seq. Assembled and annotated the transcriptome, identified differentially expressed genes, and performed functional enrichment analysis to uncover potential adaptations to the extreme environment.
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Utilized bioinformatics tools, including FASTQC, Trimmomatic, and HISAT2, to process and align RNA-seq reads to the reference genome.
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Employed differential expression analysis software, such as DESeq2 and edgeR, to identify genes with significant changes in expression between different experimental conditions or groups.
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Performed functional enrichment analysis using tools like Gene Ontology and KEGG pathway analysis to elucidate the biological processes and pathways affected by the differentially expressed genes.
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Generated comprehensive reports and visualizations to effectively communicate the findings to both scientific and… Read full answer
Source: https://hireabo.com/job/5_1_45/Bioinformatics%20Specialist
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