Sample interview questions: Can you discuss your experience with method validation for identification and quantification of unknown compounds?
Sample answer:
- Experience in Method Development and Optimization:
Demonstrate your expertise in developing analytical methods from scratch or optimizing existing ones to achieve the desired sensitivity, accuracy, and precision. Highlight your ability to select and evaluate appropriate instrumentation, reagents, and procedures to address specific analytical challenges. Showcase your experience in optimizing parameters such as extraction techniques, chromatographic conditions, and detection methods to enhance method performance.
- Proficiency in Sample Preparation Techniques:
Emphasize your proficiency in various sample preparation techniques, including liquid-liquid extraction, solid-phase extraction, and derivatization. Describe your experience in optimizing these techniques to minimize matrix effects, improve analyte recovery, and ensure the integrity of the analytical results.
- Expertise in Chromatographic Techniques:
Highlight your expertise in utilizing different chromatographic techniques, such as gas chromatography (GC) and liquid chromatography (LC), to separate and identify unknown compounds. Discuss your experience in selecting appropriate stationary phases, mobile phases, and detection methods to achieve optimal resolution and sensitivity.
- Experience in Spectroscopic Techniques:
Demonstrate your proficiency in employing various spectroscopic techniques, including mass spectrometry (MS), nuclear magnetic resonance (NMR), and infrared (IR) spectroscopy, for the identification and characterization of unknown compounds. Describe your experience in interpreting spectral data, identifyi… Read full answer