Sample interview questions: Can you discuss any experience you have with CRISPR-based gene activation or repression techniques in biotechnology research?
Sample answer:
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CRISPR-based gene activation:
- Experience in utilizing CRISPRa (CRISPR activation) systems for targeted gene overexpression.
- Proficient in designing sgRNAs (single-guide RNAs) to specifically target the promoters or regulatory regions of genes of interest.
- Hands-on experience with various CRISPRa platforms, including dCas9-VP64, dCas9-SunTag, and CRISPRi-dCas9-KRAB.
- Ability to optimize CRISPRa conditions, such as sgRNA concentration, transfection methods, and cell type selection, to achieve efficient gene activation.
- Expertise in evaluating gene activation efficiency through quantitative gene expression analysis methods, such as RT-qPCR, RNA-seq, and single-cell RNA-seq.
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CRISPR-based gene repression:
- Experience in employing CRISPRi (CRISPR interference) systems for targeted gene silencing.
- Proficient in designing sgRNAs to specifically target the coding regions or regulatory elements of genes of interest.
- Hands-on experience with various CRISPRi platforms, including dCas9-KRAB, dCas9-MS2, and CRISPRi-dCas9-SID.
- Ability to optimize CRISPRi conditions, such as sgRNA concentration, transfection methods, and cell type selection, to achieve efficient gene repression. Read full answer
Source: https://hireabo.com/job/5_1_47/Biotechnology%20Analyst