Sample interview questions: Can you discuss any experience you have with cell culture and maintenance?
Sample answer:
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Cell Culture and Maintenance Experience:
- Successfully established and maintained various cell lines, including primary human cell lines, immune cell lines, and cancer cell lines.
- Demonstrated proficiency in sterile cell culture techniques, including media preparation, cell passaging, and cell counting.
- Maintained aseptic conditions throughout all cell culture procedures to prevent contamination.
- Performed regular quality control checks on cell cultures to ensure cell viability and absence of contamination.
- Created and maintained detailed cell culture records, including cell line information, media composition, and passage history.
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Expertise in Cell Culture Techniques:
- Isolation and culture of primary human immune cells, including peripheral blood mononuclear cells (PBMCs), natural killer (NK) cells, and dendritic cells.
- Generation of dendritic cells from monocytes using various protocols.
- Culture of human tumor cell lines, including breast cancer, lung cancer, and melanoma cell lines.
- Co-culture of immune cells and tumor cells to study cell-cell interactions.
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Experience with Advanced Cell Culture Techniques:
- Performed cell sorting using fluorescence-activated cell sorting (FACS) to isolate specific cell populations.
- Conduc… Read full answer