Sample interview questions: Can you describe any experience you have in studying the energetics of molecular interactions in biological systems?
Sample answer:
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Experience in Applying Computational Methods to Study Molecular Interactions:
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Utilized molecular dynamics simulations to investigate the binding mechanisms and energetics of protein-ligand interactions.
- Employed free energy perturbation and steered molecular dynamics to quantify the binding affinities and energy landscapes of biomolecular complexes.
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Performed molecular docking studies to identify potential binding sites and predict the orientations of ligands in protein structures.
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Expertise in Experimental Techniques for Probing Molecular Interactions:
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Conducted isothermal titration calorimetry (ITC) experiments to measure the thermodynamics of protein-ligand binding, including enthalpy, entropy, and binding constants.
- Utilized surface plasmon resonance (SPR) spectroscopy to study the kinetics of molecular interactions in real-time.
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Performed fluorescence spectroscopy and Förster resonance energy transfer (FRET) assays to investigate conformational changes and protein-protein interactions.
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Experience in Collaborating with Interdisciplinary Teams:
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Collaborated with biochemists, molecular biologists, and pharmacologists to design and exec… Read full answer