Sample interview questions: Have you used molecular techniques in your ecological studies? If yes, explain how.
Sample answer:
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DNA Barcoding: I have employed DNA barcoding to identify and characterize species within various ecological communities. This technique involves extracting a short, standardized DNA sequence from a specific gene region (often the mitochondrial cytochrome c oxidase I gene) and using it to identify the organism to the species level or even finer taxonomic resolution. DNA barcoding has enabled me to assess species diversity, detect cryptic species, and uncover patterns of genetic variation within ecological communities.
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PCR-Based Assays: I have utilized PCR (polymerase chain reaction)-based assays to detect the presence or absence of specific DNA sequences associated with microorganisms, genes, or environmental contaminants in ecological samples. PCR amplifies a specific DNA region of interest, allowing for its detection and quantification. This technique has proven valuable in studying microbial diversity, assessing gene expression levels, and detecting environmental pollutants in various ecosystems.
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Quantitative Real-Time PCR (qPCR): I have implemented qPCR to quantify the abundance of specific genes, transcripts, or microbial taxa in ecological samples. qPCR employs fluorescent dyes or probes to measure the amplification of target DNA sequences during the PCR process. By monitoring the fluorescenc… Read full answer