Sample interview questions: Can you explain your familiarity with functional genomics or CRISPR/Cas9 screening data analysis using bioinformatics tools?
Sample answer:
Functional Genomics and CRISPR/Cas9 Screening Data Analysis Proficiency
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CRISPR/Cas9 Screening Data Analysis:
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Adept at processing raw sequencing reads from CRISPR/Cas9 screens using bioinformatics pipelines such as MAGeCK, CRISPRAnalyzer, and CRISPick.
- Proficiency in analyzing and interpreting sgRNA enrichment/depletion data to identify potential genetic dependencies or resistance mechanisms.
- Experienced in applying statistical methods (e.g., FDR correction, log transformation) to determine significant hits from CRISPR screens.
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Skilled in visualizing CRISPR screening data using heatmaps, volcano plots, and network analysis tools.
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Functional Genomics Data Analysis:
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Proficient in analyzing high-throughput functional genomics datasets, including RNA-Seq, microarray, and proteomics data.
- Experienced in identifying differentially expressed genes, pathways, and protein-protein interactions using bioinformatics tools such as DESeq2, Gene Set Enrichment Analysis (GSEA), and STRING.
- Adept at integrating multiple functiona… Read full answer
Source: https://hireabo.com/job/5_1_45/Bioinformatics%20Specialist