Sample interview questions: Explain your familiarity with gene silencing techniques.
Sample answer:
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RNA Interference (RNAi):
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Utilizes short interfering RNA (siRNA) or microRNA (miRNA) to target and silence specific genes.
- siRNA or miRNA molecules are designed to be complementary to the mRNA of the target gene.
- Upon binding to the mRNA, they trigger the degradation of the mRNA, preventing translation and protein production.
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This technique is widely used in functional genomics studies, drug screening, and potential therapeutic applications.
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CRISPR-Cas9 System:
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A revolutionary gene editing technique that allows precise targeting and modification of DNA sequences.
- Utilizes a guide RNA (gRNA) molecule to direct the Cas9 protein to a specific genomic location.
- Cas9 then creates a double-strand break at the target site, which can be repaired by cellular DNA repair mechanisms.
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This system enables gene knockout, gene activation, and precise gene editing, making it a powerful tool for genetic research and potential therapeutic interventions.
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Gene Silencing by Transcriptional Repression:
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Employs transcription factors or other regulatory proteins to inhibit gene transcription.
- Can be a… Read full answer