Sample interview questions: Describe your experience with molecular markers and characterization of stem cells.
Sample answer:
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Experience with Molecular Markers:
- Developed and optimized a variety of molecular markers, including microsatellites, single-nucleotide polymorphisms (SNPs), and expressed sequence tags (ESTs), for use in stem cell research.
- Utilized molecular markers to genotype stem cells, assess genetic diversity, and identify genetic markers associated with specific stem cell properties.
- Employed molecular markers to track stem cell differentiation and lineage tracing.
- Collaborated with computational biologists to develop bioinformatics tools and algorithms for analyzing molecular marker data.
- Published several papers on the use of molecular markers in stem cell research.
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Experience with Characterization of Stem Cells:
- Conducted comprehensive characterization of stem cells, including pluripotency assays, differentiation assays, and gene expression analysis.
- Utilized flow cytometry, immunocytochemistry, and other techniques to analyze stem cell surface markers, intracellular proteins, and gene expression.
- Performed functional assays to assess the differentiation potential of stem cells into various cell types.
- Developed and optimized culture conditions for the maintenance and expansion of stem cell… Read full answer