Sample interview questions: Can you discuss your experience with techniques like chromatin immunoprecipitation sequencing (ChIP-seq) or DNA methylation analysis?
Sample answer:
Chromatin Immunoprecipitation Sequencing (ChIP-seq) Experience:
- Conducted comprehensive ChIP-seq experiments to study the interactions between proteins and DNA in various biological systems.
- Optimized experimental parameters, including chromatin crosslinking, sonication conditions, and immunoprecipitation efficiency, to ensure high-quality data.
- Generated sequencing libraries using appropriate techniques, such as end-repair, adapter ligation, and library amplification, ensuring library integrity and maximizing sequencing efficiency.
- Performed data analysis, including peak calling, data normalization, and statistical analysis, to identify regions of protein-DNA interactions.
- Combined ChIP-seq data with other genomic and functional data to gain insights into gene regulation, chromatin dynamics, and disease mechanisms.
DNA Methylation Analysis Experience:
- Conducted DNA methylation analysis using bisulfite conversion and various sequencing platforms, including whole-genome bisulfite sequencing ( frontalWBS) and targeted bisulfite sequencing (TBS).
- Developed and implemented robust pipelines for data processing and analysis, including quality control, alignment, and methylation calling, ensuring accurate and reliable results.
- Analyzed DNA methylation patterns in different cell types, tissues, and disease states, uncovering epigenetic alterations associated with gene expression changes and disease development.
- Investigated the relationship between DNA methylation and other epigenetic modifications, such as histone modifications and chromatin structure, to gain a co… Read full answer