Sample interview questions: Have you developed any bioinformatics pipelines or workflows? If so, can you describe one of them?
Sample answer:
Bioinformatics Pipeline Development
Yes, I have developed several bioinformatics pipelines for various projects. One notable pipeline focuses on the analysis of RNA-sequencing (RNA-seq) data for differential gene expression analysis. This pipeline involves the following steps:
Input: High-quality RNA-seq reads in FASTQ format
Preprocessing:
1. Read quality control and filtering using FastQC or Trimmomatic
2. Reference genome mapping using a tool such as STAR or HISAT2
3. Removal of ribosomal RNA (rRNA) reads using tools like SortMeRNA or Ribocleaner
Analysis:
1. Quantification of gene expression using a tool like Salmon or Kalisto
2. Differential gene expression analysis using tools like DESeq2 or edgeR
3. Functional annotation using databases like GO and KEGG
Output: Read full answer
Source: https://hireabo.com/job/5_1_45/Bioinformatics%20Specialist