Sample interview questions: How comfortable are you with using CRISPR-based techniques to study non-coding RNAs and their functions in cells?
Sample answer:
As a cell biologist with extensive experience in CRISPR-based techniques, I am highly comfortable utilizing these methods to study non-coding RNAs and their functions in cells.
CRISPR-based approaches offer a powerful toolset for non-coding RNA research due to their ability to:
- Precisely target specific non-coding RNA sequences: By designing guide RNAs that target specific non-coding RNA molecules, CRISPR-Cas systems can be directed to edit or regulate these RNAs.
- Modulate non-coding RNA expression levels: CRISPR can be used to knock down or overexpress non-coding RNAs, allowing researchers to investigate their functional roles.
- Identify non-coding RNA targets: By creating CRISPR libraries that target different non-coding RNA sequences, researchers can perform genetic screens to identify downstream targets regulated by these RNAs.
- Study non-coding RNA function in vivo: By delivering CRISPR components directly into animal models, researchers can … Read full answer