Sample interview questions: Have you used molecular biology techniques, such as PCR or gene expression analysis, in your research?
Sample answer:
- Yes, I have extensively utilized molecular biology techniques, including PCR and gene expression analysis, in my immunology research.
PCR (Polymerase Chain Reaction):
- I have employed PCR to amplify specific DNA sequences of interest.
- This technique enables me to study the presence, quantity, or genetic variations of specific genes or DNA regions.
- In my research, I have used PCR to:
- Detect and quantify infectious agents like bacteria or viruses in clinical samples.
- Determine the genetic diversity of immune cells and identify genetic variations associated with immune disorders.
- Amplify DNA fragments for cloning and genetic engineering experiments.
Gene Expression Analysis:
- I have conducted gene expression analysis to investigate the levels of RNA transcripts corresponding to genes of interest.
- This analysis helps me understand gene regulation, changes in gene activity, and cellular responses to various stimuli.
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Methods I have used include:
- Quantitative Real-Time PCR (qPCR): Measures the amount of RNA transcripts during the PCR amplification process, allowing for accurate quantification of gene expression levels.
- Microarrays: Assays the expression levels of thousands of genes simultaneously, providing a global view of gene activity.
- RNA Sequencing (RNASeq): Read full answer