Sample interview questions: Describe the principles behind laser-induced fluorescence imaging and its applications in cancer research.
Sample answer:
Principles of Laser-Induced Fluorescence Imaging (LIF)
LIF is a non-invasive imaging technique that exploits the inherent fluorescence properties of biomarkers or dyes when stimulated by a laser. The fundamental principles involve:
- Excitation: A laser emits light of specific wavelengths that are absorbed by the fluorescent molecules or dyes, causing them to transition to an excited electronic state.
- Emission: The excited molecules spontaneously return to their ground state, releasing energy in the form of fluorescence photons that have longer wavelengths than the excitation light.
Applications in Cancer Research
LIF offers versatile applications in cancer research due to its ability to:
- Detect and Visualize Tumors: Fluorescent dyes can be injected into a patient’s body, selectively binding to cancer cells or specific biomarkers associated with tumors. LIF imaging can then be used to non-invasively visualize and map the presence and location of tumors.
- Assess Tumor Heterogeneity: LIF can distinguish between different tumor cell types based on their distinct fluorescence properties. This information can help guide personalized treatment strategies.
- Monitor Tumor Response to Therapy: LIF allows researchers to track the progression and response of tumors to var… Read full answer