How do you assess the biocompatibility and bioactivity of materials in your research?

Sample interview questions: How do you assess the biocompatibility and bioactivity of materials in your research?

Sample answer:

1. Material Characterization:
2. Analyze the chemical composition and structure of the material using techniques such as X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM).

3. Evaluate the surface properties, including topography, roughness, and wettability, using atomic force microscopy (AFM) and contact angle measurements.

4. In Vitro Biocompatibility Assessment:

5. Cytotoxicity Testing:

   • Perform cell culture assays to assess the material’s toxicity towards various cell lines.
   • Evaluate cell viability, proliferation, and morphology using assays like MTT, Alamar Blue, and LIVE/DEAD staining.

6. Protein Adsorption and Cell Adhesion:

7. Study the adsorption of proteins (e.g., fibronectin, albumin) onto the material surface using techniques like quartz crystal microbalance (QCM) and enzyme-linked immunosorbent assay (ELISA).

8. Evaluate cell adhesion and spreading on the material surface using microscopy techniques and cell adhesion assays.

9. Bioactivity Assessment:

10. Osteoinductivity:

    • Co-culture the material with osteoblast cells and assess their differentiation, proliferation, and mineralization using assays like alkaline phosphatase activity, calcium deposition, and gene expression analysis.

11. In Vivo Biocompatibility and Bioactivity Evaluation:

12. Animal Studies:

    • Implant the material into animal models (e.g., rodents, rabbits) and assess its biocompatibility and bioactivity in vivo.
    • Evaluate tiss… Read full answer

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