Sample interview questions: Can you describe any experience you have with studying cell metabolism using isotopic labeling techniques?
Sample answer:
Can you describe any experience you have with studying cells using isotopic labeling techniques?
During my postdoctoral research, I employed isotopic labeling techniques extensively to investigate protein dynamics and subcellular localization in living cells. Here are two examples that highlight my expertise:
1. Stable Isotope Labeling by Essential Nutrients (SILEN):
- Used SILEN to metabolically incorporate stable isotopes (e.g., 15N, 13C) into cellular proteins in vivo.
- Combined SILEN with mass spectrometry to monitor protein synthesis, turnover, and compartmentalization.
- Identified and quantified newly synthesized proteins during specific cellular processes (e.g., cell cycle, response to stimuli).
2. Fluorescence Isotope Labeling of Proteins (FLIP):
- Utilized FLIP to label specific… Read full answer