Sample interview questions: How do you approach studying protein aggregation and misfolding using biophysical techniques?
Sample answer:
Biophysical Techniques for Studying Protein Aggregation and Misfolding
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Spectroscopic Techniques:
- Circular Dichroism (CD): Detects changes in protein secondary structure upon aggregation.
- Fluorescence Spectroscopy: Uses fluorescent probes to monitor protein conformational changes and aggregation kinetics.
- Nuclear Magnetic Resonance (NMR): Provides atomic-level insights into protein structure and dynamics, including aggregation-induced changes.
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Microscopic Techniques:
- Atomic Force Microscopy (AFM): Visualizes protein aggregates at the nanoscale and measures their size and morphology.
- Cryo-Electron Microscopy (Cryo-EM): Determines the 3D structure of protein aggregates, providing insights into their molecular organization.
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Scattering Techniques:
- Dynamic Light Scattering (DLS): Measures the size distributi… Read full answer