Sample interview questions: Can you discuss any experience you have with bioinformatics tools and software for genomics or proteomics analysis?
Sample answer:
Bioinformatic Tools and Software Experience
As a biotechnologist, I have extensive experience with a comprehensive suite of bioinformatic tools and software for genomics and proteomic analysis.
Genomics
- Genome Assembly: I am proficient in using tools such as BWA, CLC Workbench, and SPAdes for de novo and reference-based assembly of large and complex genomes.
- Variant Analysis: I have expertise in identifying and analyzing genetic variations using GATK, VCFtools, and annovar. I have developed custom workflows for variant annotation, prioritization, and interpretation.
- Comparative Genomics: I have used tools like MEGA and OrthoMCL to perform phylogenetic analyses and identify homologous genes across different species. This experience has enabled me to understand the evolution and diversity of genomes.
Transcriptomics
- RNA-Seq Analysis: I am familiar with tools such as Tophat2, Cufflinks, and DESeq2 for analyzing RNA-Seq data. I have experience in differential gene expression analysis, splicing analysis, and isoform detection.
- Microarray Analysis: I have used platforms such as Affymetrix and Agilent microarays for gene expression studies. I am proficient in data pre-processing, quality control, and differential an… Read full answer