Sample interview questions: How do you approach designing experiments to investigate the biocompatibility of inorganic materials?
Sample answer:
Approaching the Design of Biocompatibility Experiments for Inorganic Materials:
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Model System Selection: Choose representative materials with varying properties (e.g., surface chemistry, particle size, surface area) to assess their impact on biocompatibility.
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In Vitro Testing: Utilize cell culture models to evaluate cell viability, proliferation, and differentiation. Select cells that are relevant to the intended application (e.g., osteoblasts for bone implants). Employ cytotoxicity, genotoxicity, and genotoxicity assays to assess toxicity and potential genetic damage.
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In Vivo Testing: Conduct animal studies to evaluate material performance in a physiological environment. Assess biocompatibility through histology, immunohistochemistry, and micro-computed tomography. Consider long-term implantation studies to monitor chronic responses.
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Biofunctionalization: Explore surface modifications or coatings to enhance biocompatibility. Test materials functionalized with biomolecules (e.g., peptides, proteins, polymers) to promote cell attachment, growth, and differentiation.
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Statistical Analysis: Employ rigorous statistical methods to analyze the s… Read full answer