Sample interview questions: How do you handle the analysis of biological fluids such as saliva, urine, or semen in forensic investigations?
Sample answer:
Analysis of Biological Fluids in Forensic Investigations
- Sample Collection:
- Collect samples using sterile containers and proper protective gear to avoid contamination.
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Document the time, location, and circumstances of collection.
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Preservation and Storage:
- Store samples refrigerated or frozen to maintain DNA integrity.
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Utilize appropriate preservatives to prevent degradation.
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Screening:
- Perform preliminary tests to identify the type of fluid (e.g., ABO grouping, specific gravity).
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Employ immunoassays to detect the presence of specific substances, such as blood or semen.
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DNA Extraction:
- Extract DNA from the fluid using specialized techniques to obtain high-quality genetic material.
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Ensure purity and yield to minimize analysis errors.
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Quantification:
- Determine the amount of DNA present in the sample using spectrophotometry or quantitative PCR.
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Estimate the potential quantity of genetic information available for analysis.
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Profiling:
- Amplify and analyze DNA using mul… Read full answer