Sample interview questions: Tell us about your familiarity with techniques used in studying cell migration and invasion in 2D and 3D models.
Sample answer:
2D Models:
- Wound healing assays: Measure cell migration into a “scratch” created in a confluent cell monolayer.
- Boyden chamber assays: Quantify cell migration through a porous membrane.
- Transwell assays: Assess cell invasion through a Matrigel or collagen-coated membrane.
3D Models:
- Spheroid assays: Measure cell invasion and outgrowth from 3D spheroids embedded in a matrix.
- Organotypic slice cultures: Use tissue slices from target organs to study cell migration and invasion in a physiologically relevant context.
- Microfluidic devices: Create controlled environments to study cell migration and invasion in response to gradients of cu… Read full answer