Tell us about your familiarity with techniques used in studying cell migration and invasion in 2D and 3D models.

Sample interview questions: Tell us about your familiarity with techniques used in studying cell migration and invasion in 2D and 3D models.

Sample answer:

2D Models:

  • Wound healing assays: Measure cell migration into a “scratch” created in a confluent cell monolayer.
  • Boyden chamber assays: Quantify cell migration through a porous membrane.
  • Transwell assays: Assess cell invasion through a Matrigel or collagen-coated membrane.

3D Models:

  • Spheroid assays: Measure cell invasion and outgrowth from 3D spheroids embedded in a matrix.
  • Organotypic slice cultures: Use tissue slices from target organs to study cell migration and invasion in a physiologically relevant context.
  • Microfluidic devices: Create controlled environments to study cell migration and invasion in response to gradients of cu… Read full answer

    Source: https://hireabo.com/job/5_1_9/Cell%20Biologist

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