Sample interview questions: Can you discuss your experience with techniques like two-hybrid systems or protein-protein interaction assays?
Sample answer:
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Two-hybrid systems:
- Experience in constructing two-hybrid libraries and screening for protein-protein interactions
- Proficient in using reporter genes to detect protein-protein interactions
- Expertise in optimizing two-hybrid assays to increase sensitivity and reduce false positives
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Protein-protein interaction assays:
- Experience in conducting a variety of protein-protein interaction assays, including co-immunoprecipitation, GST pull-down, and fluorescence resonance energy transfer (FRET)
- Proficient in using these assays to study protein-protein interactions in vitro and in vivo
- Expertise in optimizing protein-protein interaction assays to increase sensitivity and specificity
Additional skills and experience: