Sample interview questions: Have you ever used proteomics to study protein folding, structure, or dynamics?
Sample answer:
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Protein Folding Studies:
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Utilized proteomics techniques to examine protein folding pathways and conformational changes in response to various factors, such as temperature, pH, and ligand binding.
- Employed hydrogen-deuterium exchange coupled with mass spectrometry to monitor folding intermediates, unfolding kinetics, and conformational stability.
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Explored the impact of mutations or post-translational modifications on protein folding processes.
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Protein Structure Analysis:
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Applied proteomic approaches to characterize protein structures, including secondary and tertiary structures, as well as interactions with other molecules.
- Used limited proteolysis and mass spectrometry to map protein domains, cleavage sites, and conformational changes.
- Performed chemical cross-linking coupled with mass spectrometry to elucidate protein-protein interactions and structural networks.
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Utilized protein interaction studies to gain insights into protein complexes and assemblies.
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Protein Dynamics Investigations:
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Implemented proteomics techniques to study protein dynamics, such as conformational fluctuations, allosteric transitions, and enzy… Read full answer
Source: https://hireabo.com/job/5_1_42/Proteomics%20Scientist