Sample interview questions: Explain your experience with protein characterization and functional assays.
Sample answer:
- Protein Expression and Purification:
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Expertise in expressing and purifying proteins using various systems, including bacterial, yeast, insect, and mammalian cell culture.
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Protein Characterization Techniques:
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Extensive experience in protein characterization techniques such as SDS-PAGE, Western blotting, immunoprecipitation, and protein-protein interaction assays.
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Functional Assays:
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Demonstrated ability to develop and perform functional assays to assess protein activity, including enzyme activity assays, binding assays, and cell-based assays.
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Protein Structure Analysis:
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Experience in protein structure analysis using techniques such as X-ray crystallography and NMR spectroscopy.
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Protein Interaction Studies:
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Expertise in studying protein-protein interactions using techniques such as co-immunoprecipitation, cross-linking, and yeast two-hybrid assays.
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Protein Engineering and Mutagenesis:
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Experience in protein engineering and mutagenesis to generate variants… Read full answer