Sample interview questions: Can you discuss any experience you have with molecular cloning techniques?
Sample answer:
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Experience with molecular cloning techniques:
- Constructed recombinant DNA molecules using restriction enzymes, ligases, and other molecular biology techniques.
- Transformed bacteria and yeast cells with recombinant DNA molecules.
- Selected and screened for transformants containing the desired recombinant DNA molecules.
- Analyzed the structure and function of recombinant DNA molecules using a variety of techniques, including DNA sequencing, Southern blotting, and PCR.
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Skills and knowledge:
- Proficient in a variety of molecular cloning techniques, including restriction enzyme digestion, ligation, transformation, and PCR.
- Strong understanding of the principles of molecular biology and genetics.
- Ability to design and execute experiments to investigate gene expression and regulation.
- Excellent laboratory skills and attention to detail.
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Experience with specific molecular cloning techniques:
- Restriction enzyme digestion: Used restriction enzymes to cut DNA at specific sequences.
- Ligation: Used ligases to join DNA fragments together.
- Transformation: Transformed bacteria and yeast cells with recombinant DNA molecules.
- PCR: Used PCR to amplify DNA fragments.
- DNA sequencing: Used DNA sequencing to determine the sequence of DNA molecules.
- Southern blotting: Read full answer