Sample interview questions: Tell us about any experience you have with genome-wide screening techniques in cell biology.
Sample answer:
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CRISPR-Cas9 genome editing: I have extensive experience utilizing CRISPR-Cas9 for genome-wide screening in human cell lines and animal models. I have successfully generated libraries of guide RNAs targeting essential genes and conducted high-throughput screening to identify genes critical for cell viability and function.
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RNA-sequencing (RNA-seq): I am proficient in performing RNA-seq for genome-wide gene expression profiling. I have analyzed RNA-seq data to identify differentially expressed genes in response to various treatments or genetic perturbations. I have also used RNA-seq to characterize the transcriptional landscape of cell types and disease states.
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ATAC-sequencing (ATAC-seq): I have experience in performing ATAC-seq to map genome-wide chromatin accessibility. I have utilized ATAC-seq to identify regulatory elements, such as enhancers and promoters, and to study the epigenetic regulation of gene expression.
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Single-cell RNA-seq (scRNA-seq): I am skilled in performing scRNA-seq to analyze gene expression at the single-cell level. I have used scRNA-seq to identify … Read full answer